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TerryDR QuPath PhenoCluster + Spa Q&A

Views: 0     Author: Site Editor     Publish Time: 2025-07-16      Origin: Site

Inquire

Q1: What can TerryDR PhenoCluster + Spa do?
A: PhenoCluster + Spa can complete three core tasks within 10 minutes:


  1. Cell phenotype analysis: Automatically parse 60+ protein markers in multiplex immunofluorescence / flow mass spectrometry images, and accurately identify lymphocytes and their subsets, tumor cells and other types.

  2. Spatial interaction modeling: Quantify cell neighborhood relationships (such as immune cell - tumor distance), immune exclusion zones and infiltration hotspots.

  3. Human expert - level output: Reach the level of traditional manual analysis. One click directly generates pathological spatial biology analysis reports, while Halo / QuPath takes 3 hours.
    It is equivalent to upgrading pathological analysis from "manual carving" to "industrial - grade 3D printing".


Q2: Why is the efficiency and analysis speed of TerryDR PhenoCluster + Spa so high?
A: The core breakthrough lies in the pre-trained AI engine:


  • 200 million cell annotation database: Trained based on mIF (multiplex immunofluorescence) and CyTOF (imaging mass spectrometry) images, accumulating 10,000 times more experience than human experts.

  • Skip the trap of manual parameter adjustment: Traditional methods need to repeatedly fine - tune threshold classifiers or Object classifiers. PhenoCluster directly outputs results based on AI, avoiding human errors.

  • Zero - configuration startup: Out - of - the - box models save more than 95% of operation steps.
    It is like replacing manual driving with Tesla autopilot technology.


Q3: How does TerryDR PhenoCluster + Spa make spatial biology analysis so simple?
A: Through a three - step minimalist operation chain:


  1. Annotate the analysis region (ROI) and segment cells in QuPath (QuPath native function)

  2. Input the predefined rule table (for example: CD3 + CD4 + = Th cells)

  3. Automatically generate cell phenotype analysis results and spatial interaction maps (such as cell neighborhood networks, immune exclusion heat maps)
    Master it proficiently in 15 minutes without algorithm background.


Q4: What platform data can TerryDR PhenoCluster + Spa analyze?
A: Fully compatible with mainstream platforms (99% of brands and methods on the market):
Platform type _ Representative platforms
All
Multiplex fluorescence staining _ PhenoCycler (Akoya), Comet (Lunaphore), IBEX
Imaging mass spectrometry _ Hyperion tissue flow mass spectrometer
Extended support _ Visium spatial transcriptome (may require StainSync registration)
Build cross - platform unified analysis standards.


Q5: In addition to the PhenoCluster + Spa plug-in, what other QuPath plug-ins has TerryDR developed?
A: The golden triangle of plug-ins:


  1. StainSync:


  • Complete multi - modal slice registration (H&E / IHC / mIF / Visium) in 5 minutes

  • Solve the core challenge faced by most fluorescence scanners on the market: unable to achieve spatial alignment of multi - round cyclic staining images, such as image displacement deviation and deformation distortion.


  1. CellSage:


  • Automatically classify H&E slices into 5 categories: tumor cells, immune cells, dead cells, etc.

  • Transformer architecture pre - trained model, zero annotation startup.


  1. PhenoCluster + Spa:


  • Undertake the previous results and output spatial biology reports.
    Form a fully automatic pipeline of "registration → classification → analysis".


Q6: What is the TerryDR CellSage plug-in used for?
A: A revolutionary tool for H&E analysis:


  • Dual core capabilities:
    Cell segmentation: Accurately locate each cell in the tissue.
    Intelligent classification: Identify tumor cells, inflammatory cells, connective tissues, etc.

  • Seamless connection with spatial analysis:
    Output cell coordinates + types, directly connect to the Spa plug-in to generate spatial interaction networks (such as TILs dynamic distribution maps).

  • Support for clinical diagnosis:
    Support manual correction and multi - person annotation to ensure the consistency of results.


Domain clustering
Cell connection
Square correlation matrix
Square area


Value summary
TerryDR plug-in ecosystem
The "intelligent operating system" of spatial biology:


  • 10 times efficiency improvement: AI replaces manual threshold fine - tuning.

  • Support for precision medicine: Spatial heterogeneity index guides treatment decisions.

  • Platform unbounded: Compatible with more than 20 devices to build unified analysis standards.


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